Ontology highlight
ABSTRACT:
SUBMITTER: Yamada S
PROVIDER: S-EPMC3562762 | biostudies-literature | 2013 Jan
REPOSITORIES: biostudies-literature
Yamada Seiji S Bouley Ford Nicole D ND Keller Gretchen E GE Ford William C WC Gray Harry B HB Winkler Jay R JR
Proceedings of the National Academy of Sciences of the United States of America 20130114 5
We have investigated the folding dynamics of Thermus thermophilus cytochrome c(552) by time-resolved fluorescence energy transfer between the heme and each of seven site-specific fluorescent probes. We have found both an equilibrium unfolding intermediate and a distinct refolding intermediate from kinetics studies. Depending on the protein region monitored, we observed either two-state or three-state denaturation transitions. The unfolding intermediate associated with three-state folding exhibit ...[more]