Ontology highlight
ABSTRACT:
SUBMITTER: Han SO
PROVIDER: S-EPMC152600 | biostudies-literature | 2003 Apr
REPOSITORIES: biostudies-literature
Han Sung Ok SO Yukawa Hideaki H Inui Masayuki M Doi Roy H RH
Journal of bacteriology 20030401 8
Transcription of the cellulosomal cellulase/hemicellulase genes of Clostridium cellulovorans has been investigated by Northern blot, reverse transcriptase PCR (RT-PCR), primer extension, and S1 nuclease analysis. Northern hybridizations revealed that the cellulosomal cbpA gene cluster is transcribed as polycistronic mRNAs of 8 and 12 kb. The 8-kb mRNA coded for cbpA and exgS, and the 12-kb mRNA coded for cbpA, exgS, engH, and engK. The sizes of the mRNAs were about 3 kb for engE, 1.8 kb for manA ...[more]