Ontology highlight
ABSTRACT:
SUBMITTER: Murashima K
PROVIDER: S-EPMC1251607 | biostudies-literature | 2005 Oct
REPOSITORIES: biostudies-literature
Murashima Koichiro K Kosugi Akihiko A Doi Roy H RH
Journal of bacteriology 20051001 20
The planar and anchoring residues of the family IIIa cellulose binding domain (CBD) from the cellulosomal scaffolding protein of Clostridium cellulovorans were investigated by site-directed mutagenesis and cellulose binding studies. By fusion with maltose binding protein, the family IIIa recombinant wild-type and mutant CBDs from C. cellulovorans were expressed as soluble forms. Cellulose binding tests of the mutant CBDs indicated that the planar strip residues played a major role in cellulose b ...[more]