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Profile of native cellulosomal proteins of Clostridium cellulovorans adapted to various carbon sources.

ABSTRACT: We performed a focused proteome analysis of cellulosomal proteins predicted by a genome analysis of Clostridium cellulovorans [Tamaru, Y., et al.. 2010. J. Bacteriol. 192:901-902]. Our system employed a long monolithic column (300?cm), which provides better performance and higher resolution than conventional systems. Twenty-three cellulosomal proteins were, without purification, identified by direct analysis of the culture medium. Proteome analysis of the C. cellulovorans cellulosome after culture in various carbon sources demonstrated the production of carbon source-adapted cellulosome components.

SUBMITTER: Morisaka H

PROVIDER: S-EPMC3444338 | biostudies-literature | 2012 Jul

REPOSITORIES: biostudies-literature

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Profile of native cellulosomal proteins of Clostridium cellulovorans adapted to various carbon sources.

Morisaka Hironobu H Matsui Kazuma K Tatsukami Yohei Y Kuroda Kouichi K Miyake Hideo H Tamaru Yutaka Y Ueda Mitsuyoshi M

AMB Express 20120729 1

We performed a focused proteome analysis of cellulosomal proteins predicted by a genome analysis of Clostridium cellulovorans [Tamaru, Y., et al.. 2010. J. Bacteriol. 192:901-902]. Our system employed a long monolithic column (300 cm), which provides better performance and higher resolution than conventional systems. Twenty-three cellulosomal proteins were, without purification, identified by direct analysis of the culture medium. Proteome analysis of the C. cellulovorans cellulosome after cultu ...[more]

PMID: 22839966

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