Ontology highlight
ABSTRACT:
SUBMITTER: Chavatte L
PROVIDER: S-EPMC152891 | biostudies-literature | 2003 Apr
REPOSITORIES: biostudies-literature
Chavatte Laurent L Kervestin Stéphanie S Favre Alain A Jean-Jean Olivier O
The EMBO journal 20030401 7
During eukaryotic translation termination, eRF1 responds to three stop codons. However, in ciliates with variant genetic codes, only one or two codons function as a stop signal. To localize the region of ciliate eRF1 implicated in stop codon discrimination, we have constructed ciliate-human hybrid eRF1s by swapping regions of human eRF1 for the equivalent region of ciliate Euplotes eRF1. We have examined the formation of a cross-link between recombinant eRF1s and mRNA analogs containing the phot ...[more]