Role of the AP2 beta-appendage hub in recruiting partners for clathrin-coated vesicle assembly.

ABSTRACT: Adaptor protein complex 2 alpha and beta-appendage domains act as hubs for the assembly of accessory protein networks involved in clathrin-coated vesicle formation. We identify a large repertoire of beta-appendage interactors by mass spectrometry. These interact with two distinct ligand interaction sites on the beta-appendage (the "top" and "side" sites) that bind motifs distinct from those previously identified on the alpha-appendage. We solved the structure of the beta-appendage with a peptide from the accessory protein Eps15 bound to the side site and with a peptide from the accessory cargo adaptor beta-arrestin bound to the top site. We show that accessory proteins can bind simultaneously to multiple appendages, allowing these to cooperate in enhancing ligand avidities that appear to b

SUBMITTER: Schmid EM 

PROVIDER: S-EPMC1540706 | biostudies-literature | 2006 Sep

REPOSITORIES: biostudies-literature