Unknown

Dataset Information

0

Transcriptional regulation of human MAP2 gene in melanoma: role of neuronal bHLH factors and Notch1 signaling.


ABSTRACT: Microtubule-associated protein 2 (MAP2), a neuron-specific protein, stabilizes microtubules and is critical for neurite outgrowth and dendrite development. Although MAP2 is widely used as a marker of neuronal differentiation, regulation of its transcription has not been investigated. We showed that MAP2 is frequently activated in human cutaneous melanoma. Here, we identified a 2.2 kb region that is sufficient for neuronal-specific expression in vitro and in vivo. Comparative analysis of the mouse, rat and human MAP2 promoter sequences showed the presence of a conserved bHLH factor binding sites. Electrophoretic mobility shift analysis, promoter mutagenesis and co-transfection experiments showed that NeuroD, a pro-neuronal differentiation factor, and Hairy and Enhancer of Split (HES1), a transcription repressor, are involved in the regulation of MAP2 promoter activity. Melanoma cells express both NeuroD and HES1. Chromatin immunoprecipitation showed that in metastatic melanoma cells N-box region of the MAP2 promoter is occupied by endogenous HES1. We show that the inhibition of Notch signaling, a regulator of HES1 gene expression, and/or shRNA knockdown of HES1 results in the upregulation of MAP2 promoter activity. Thus, our data suggest that Notch signaling, which is implicated in melanoma progression, and HES1 play a role in MAP2 gene regulation during melanoma progression.

SUBMITTER: Bhat KM 

PROVIDER: S-EPMC1540725 | biostudies-literature | 2006

REPOSITORIES: biostudies-literature

altmetric image

Publications

Transcriptional regulation of human MAP2 gene in melanoma: role of neuronal bHLH factors and Notch1 signaling.

Bhat Kumar M R KM   Maddodi Nityanand N   Shashikant Cooduvalli C   Setaluri Vijayasaradhi V  

Nucleic acids research 20060811 13


Microtubule-associated protein 2 (MAP2), a neuron-specific protein, stabilizes microtubules and is critical for neurite outgrowth and dendrite development. Although MAP2 is widely used as a marker of neuronal differentiation, regulation of its transcription has not been investigated. We showed that MAP2 is frequently activated in human cutaneous melanoma. Here, we identified a 2.2 kb region that is sufficient for neuronal-specific expression in vitro and in vivo. Comparative analysis of the mous  ...[more]

Similar Datasets

| S-EPMC8495403 | biostudies-literature
| S-EPMC8217713 | biostudies-literature
| S-EPMC6529535 | biostudies-literature
| S-EPMC5536923 | biostudies-literature
| S-EPMC4589600 | biostudies-literature
| S-EPMC2872778 | biostudies-literature
| S-EPMC3967047 | biostudies-literature
| S-EPMC9306894 | biostudies-literature
| S-EPMC4201386 | biostudies-literature
| S-EPMC2804171 | biostudies-other