Production of native-type Streptoverticillium mobaraense transglutaminase in Corynebacterium glutamicum.
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PROVIDER: S-EPMC154552 | biostudies-literature | 2003 May
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Production of native-type Streptoverticillium mobaraense transglutaminase in Corynebacterium glutamicum.
Date Masayo M Yokoyama Kei-ichi K Umezawa Yukiko Y Matsui Hiroshi H Kikuchi Yoshimi Y
Applied and environmental microbiology 20030501 5
We previously observed secretion of active-form transglutaminase in Corynebacterium glutamicum by coexpressing the subtilisin-like protease SAM-P45 from Streptomyces albogriseolus to process the prodomain. However, the N-terminal amino acid sequence of the transglutaminase differed from that of the native Streptoverticillium mobaraense enzyme. In the present work we have used site-directed mutagenesis to generate an optimal SAM-P45 cleavage site in the C-terminal region of the prodomain. As a re ...[more]
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