Ontology highlight
ABSTRACT:
SUBMITTER: Fulconis R
PROVIDER: S-EPMC1570433 | biostudies-literature | 2006 Sep
REPOSITORIES: biostudies-literature
Fulconis Renaud R Mine Judith J Bancaud Aurélien A Dutreix Marie M Viovy Jean-Louis JL
The EMBO journal 20060831 18
The mechanisms of RecA-mediated three-strand homologous recombination are investigated at the single-molecule level, using magnetic tweezers. Probing the mechanical response of DNA molecules and nucleoprotein filaments in tension and in torsion allows a monitoring of the progression of the exchange in real time, both from the point of view of the RecA-bound single-stranded DNA and from that of the naked double-stranded DNA (dsDNA). We show that strand exchange is able to generate torsion even al ...[more]