Ontology highlight
ABSTRACT:
SUBMITTER: Fan HF
PROVIDER: S-EPMC3134461 | biostudies-literature | 2011
REPOSITORIES: biostudies-literature
Fan Hsiu-Fang HF Cox Michael M MM Li Hung-Wen HW
PloS one 20110712 7
RecA recombinases play a central role in homologous recombination. Once assembled on single-stranded (ss) DNA, RecA nucleoprotein filaments mediate the pairing of homologous DNA sequences and strand exchange processes. We have designed two experiments based on tethered particle motion (TPM) to investigate the fates of the invading and the outgoing strands during E. coli RecA-mediated pairing and strand exchange at the single-molecule level in the absence of force. TPM experiments measure the tet ...[more]