Ontology highlight
ABSTRACT:
SUBMITTER: Rostamighalehjaghi S
PROVIDER: S-EPMC3481692 | biostudies-literature | 2010
REPOSITORIES: biostudies-literature
Rostamighalehjaghi S S Jamali R R Rezaie S S Babaei Z Z Hooshyar H H Rezaeian M M
Iranian journal of public health 20101231 4
<h4>Background</h4>We examined a molecular method with a single-PCR for amplification of a part of CP5 gene enabling us to differentiate the pathogenic species, Entamoeba histolytica, from the non-pathogenic species, E. dispar.<h4>Methods</h4>We developed a single PCR method for this purpose. After investigation of GenBank, primer pairs were designed from highly conserved regions of cysteine proteinase (CP5) gene. The primers were utilized in PCR using isolated genomic DNA template of E. histoly ...[more]