Genetic characterization of the phenylacetyl-coenzyme A oxygenase from the aerobic phenylacetic acid degradation pathway of Escherichia coli.
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ABSTRACT:
SUBMITTER: Fernandez C
PROVIDER: S-EPMC1636149 | biostudies-literature | 2006 Nov
REPOSITORIES: biostudies-literature
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Genetic characterization of the phenylacetyl-coenzyme A oxygenase from the aerobic phenylacetic acid degradation pathway of Escherichia coli.
Fernández Cristina C Ferrández Abel A Miñambres Baltasar B Díaz Eduardo E García José L JL
Applied and environmental microbiology 20060922 11
We show here that the paaABCDE genes of the paa cluster responsible for phenylacetate degradation in Escherichia coli W encode a five-component oxygenase that hydroxylates phenylacetyl-coenzyme A (CoA), the first intermediate of the pathway. The primary structure of the subunits of bacterial phenylacetyl-CoA oxygenases revealed that these enzymes constitute the prototype of a new and distinct group of the large bacterial diiron multicomponent oxygenase family. ...[more]
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