Ontology highlight
ABSTRACT:
SUBMITTER: Jiang ZY
PROVIDER: S-EPMC164627 | biostudies-literature | 2003 Jun
REPOSITORIES: biostudies-literature
Jiang Zhen Y ZY Zhou Qiong L QL Coleman Kerri A KA Chouinard My M Boese Queta Q Czech Michael P MP
Proceedings of the National Academy of Sciences of the United States of America 20030613 13
Glucose homeostasis is controlled by insulin in part through the translocation of intracellular glucose transporter 4 to the plasma membrane in muscle and fat cells. Akt/protein kinase B downstream of phosphatidylinositol 3-kinase has been implicated in this insulin-signaling pathway, but results with a variety of reagents including Akt1-/- and Akt2-/- mice have been equivocal. Here we report the application of small interfering RNA-directed gene silencing to deplete both Akt1 and Akt2 in cultur ...[more]