Ontology highlight
ABSTRACT:
SUBMITTER: Merritt J
PROVIDER: S-EPMC169973 | biostudies-literature | 2003 Aug
REPOSITORIES: biostudies-literature
Merritt Joshua J DiTonno Jason R JR Mitra Robi D RD Church George M GM Edwards Jeremy S JS
Nucleic acids research 20030801 15
We describe a strategy to analyze the impact of single nucleotide mutations on protein function. Our method utilizes a combination of yeast functional complementation, growth competition of mutant pools and polyacrylamide gel immobilized PCR. A system was constructed in which the yeast PGK1 gene was expressed from a plasmid-borne copy of the gene in a PGK1 deletion strain of Saccharomyces cerevisiae. Using this system, we demonstrated that the enrichment or depletion of PGK1 point mutants from a ...[more]