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Integration site for Streptomyces phage phiBT1 and development of site-specific integrating vectors.

ABSTRACT: Despite extensive similarities between the genomes of the Streptomyces temperate phages phiC31 and phiBT1, the attP-int loci are poorly conserved. Here we demonstrate that phiBT1 integrates into a different attachment site than phiC31. phiBT1 attB lies within SCO4848 encoding a 79-amino-acid putative integral membrane protein. Integration vectors based on phiBT1 integrase were shown to have a broad host range and are fully compatible with those based on the phiC31 attP-int locus.

SUBMITTER: Gregory MA

PROVIDER: S-EPMC180994 | biostudies-literature | 2003 Sep

REPOSITORIES: biostudies-literature

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Integration site for Streptomyces phage phiBT1 and development of site-specific integrating vectors.

Gregory Matthew A MA Till Rob R Smith Margaret C M MC

Journal of bacteriology 20030901 17

Despite extensive similarities between the genomes of the Streptomyces temperate phages phiC31 and phiBT1, the attP-int loci are poorly conserved. Here we demonstrate that phiBT1 integrates into a different attachment site than phiC31. phiBT1 attB lies within SCO4848 encoding a 79-amino-acid putative integral membrane protein. Integration vectors based on phiBT1 integrase were shown to have a broad host range and are fully compatible with those based on the phiC31 attP-int locus. ...[more]

PMID: 12923110

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