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Differentiation of embryonic stem cells is induced by GATA factors.

ABSTRACT: Extraembryonic endoderm (ExE) is differentiated from the inner cell mass of the late blastocyst-stage embryo to form visceral and parietal endoderm, both of which have an important role in early embryogenesis. The essential roles of Gata-6 and Gata-4 on differentiation of visceral endoderm have been identified by analyses of knockout mice. Here we report that forced expression of either Gata-6 or Gata-4 in embryonic stem (ES) cells is sufficient to induce the proper differentiation program towards ExE. We believe that this is the first report of a physiological differentiation event induced by the ectopic expression of a transcription factor in ES cells.

SUBMITTER: Fujikura J

PROVIDER: S-EPMC186328 | biostudies-literature | 2002 Apr

REPOSITORIES: biostudies-literature

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Differentiation of embryonic stem cells is induced by GATA factors.

Fujikura Junji J Yamato Eiji E Yonemura Shigenobu S Hosoda Kiminori K Masui Shinji S Nakao Kazuwa K Miyazaki Ji Jun-ichi J Niwa Hitoshi H

Genes & development 20020401 7

Extraembryonic endoderm (ExE) is differentiated from the inner cell mass of the late blastocyst-stage embryo to form visceral and parietal endoderm, both of which have an important role in early embryogenesis. The essential roles of Gata-6 and Gata-4 on differentiation of visceral endoderm have been identified by analyses of knockout mice. Here we report that forced expression of either Gata-6 or Gata-4 in embryonic stem (ES) cells is sufficient to induce the proper differentiation program towar ...[more]

PMID: 11937486

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