Ontology highlight
ABSTRACT:
SUBMITTER: Tani M
PROVIDER: S-EPMC1868537 | biostudies-literature | 2007 Apr
REPOSITORIES: biostudies-literature
Tani Motohiro M Hannun Yusuf A YA
FEBS letters 20070301 7
Neutral sphingomyelinase 2 (nSMase2), which has two hydrophobic segments at its NH(2)-terminus, plays an important role in ceramide-mediated cell regulation. Here, we investigated the membrane topology of nSMase2. When a double-tagged nSMase2 at both the NH(2) and COOH termini, was overexpressed in MCF-7 cells, the signals from both tags were detected in the inner leaflet of the plasma membrane. Furthermore, insertion of a tag into the internal sequence and green fluorescent protein-fused deleti ...[more]