Ontology highlight
ABSTRACT:
SUBMITTER: Romi E
PROVIDER: S-EPMC1885581 | biostudies-literature | 2007 May
REPOSITORIES: biostudies-literature
Proceedings of the National Academy of Sciences of the United States of America 20070509 21
Telomerase is a cellular reverse transcriptase, which utilizes an integral RNA template to extend single-stranded telomeric DNA. We used site-specific photocrosslinking to map interactions between DNA primers and the catalytic protein subunit (tTERT) of Tetrahymena thermophila telomerase in functional enzyme complexes. Our assays reveal contact of the single-stranded DNA adjacent to the primer-template hybrid and tTERT residue W187 at the periphery of the N-terminal domain. This contact was dete ...[more]