RNA conformation in catalytically active human telomerase.
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ABSTRACT:
SUBMITTER: Yeoman JA
PROVIDER: S-EPMC3119468 | biostudies-literature | 2010 Mar
REPOSITORIES: biostudies-literature
Publications
RNA conformation in catalytically active human telomerase.
Yeoman Justin A JA Orte Angel A Ashbridge Beth B Klenerman David D Balasubramanian Shankar S
Journal of the American Chemical Society 20100301 9
We have used single-molecule fluorescence microscopy to study the folded state of human telomerase RNA (hTR). Here we show that hTR adopts a new conformation on binding to human telomerase reverse transcriptase (hTERT) and reconstitution of an active ribonucleoprotein complex. Our data are consistent with the formation of an RNA pseudoknot in active human telomerase. ...[more]
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