Ontology highlight
ABSTRACT:
SUBMITTER: Qi X
PROVIDER: S-EPMC3252576 | biostudies-literature | 2012 Jan
REPOSITORIES: biostudies-literature
Qi Xiaodong X Xie Mingyi M Brown Andrew F AF Bley Christopher J CJ Podlevsky Joshua D JD Chen Julian J-L JJ
The EMBO journal 20111011 1
Telomerase synthesizes telomeric DNA repeats onto chromosome termini from an intrinsic RNA template. The processive synthesis of DNA repeats relies on a unique, yet poorly understood, mechanism whereby the telomerase RNA template translocates and realigns with the DNA primer after synthesizing each repeat. Here, we provide evidence that binding of the realigned RNA/DNA hybrid by the active site is an essential step for template translocation. Employing a template-free human telomerase system, we ...[more]