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Nanoprobes with near-infrared persistent luminescence for in vivo imaging.

ABSTRACT: Fluorescence is increasingly used for in vivo imaging and has provided remarkable results. Yet this technique presents several limitations, especially due to tissue autofluorescence under external illumination and weak tissue penetration of low wavelength excitation light. We have developed an alternative optical imaging technique by using persistent luminescent nanoparticles suitable for small animal imaging. These nanoparticles can be excited before injection, and their in vivo distribution can be followed in real-time for more than 1 h without the need for any external illumination source. Chemical modification of the nanoparticles' surface led to lung or liver targeting or to long-lasting blood circulation. Tumor mass could also be identified on a mouse model.

SUBMITTER: le Masne de Chermont Q

PROVIDER: S-EPMC1890483 | biostudies-literature | 2007 May

REPOSITORIES: biostudies-literature

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Nanoprobes with near-infrared persistent luminescence for in vivo imaging.

le Masne de Chermont Quentin Q Chanéac Corinne C Seguin Johanne J Pellé Fabienne F Maîtrejean Serge S Jolivet Jean-Pierre JP Gourier Didier D Bessodes Michel M Scherman Daniel D

Proceedings of the National Academy of Sciences of the United States of America 20070521 22

Fluorescence is increasingly used for in vivo imaging and has provided remarkable results. Yet this technique presents several limitations, especially due to tissue autofluorescence under external illumination and weak tissue penetration of low wavelength excitation light. We have developed an alternative optical imaging technique by using persistent luminescent nanoparticles suitable for small animal imaging. These nanoparticles can be excited before injection, and their in vivo distribution ca ...[more]

PMID: 17517614

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