Ontology highlight
ABSTRACT:
SUBMITTER: Lee JH
PROVIDER: S-EPMC1891724 | biostudies-literature | 2007
REPOSITORIES: biostudies-literature
Lee Joon-Hwa JH Pardi Arthur A
Nucleic acids research 20070416 9
The thermodynamics and kinetics for base-pair opening of the P1 duplex of the Tetrahymena group I ribozyme were studied by NMR hydrogen exchange experiments. The apparent equilibrium constants for base pair opening were measured for most of the imino protons in the P1 duplex using the base catalysts NH3, HPO4(2-) or TRIS. These equilibrium constants were also measured for several modified P1 duplexes, and the C-2.G23 base pair was the most stable base pair in all the duplexes. The conserved U-1* ...[more]