Regulation of RKIP binding to the N-region of the Raf-1 kinase.
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ABSTRACT:
SUBMITTER: Park S
PROVIDER: S-EPMC1892598 | biostudies-literature | 2006 Nov
REPOSITORIES: biostudies-literature
Publications
Regulation of RKIP binding to the N-region of the Raf-1 kinase.
Park Sungdae S Rath Oliver O Beach Sandy S Xiang Xiaoqin X Kelly Sharon M SM Luo Zhijun Z Kolch Walter W Yeung Kam C KC
FEBS letters 20061103 27
The Raf kinase inhibitory protein (RKIP) binds to Raf-1 interfering with binding of the MEK substrate and potentially also Raf-1 activation. In response to mitogen stimulation RKIP dissociates from Raf-1 and later re-associates. Here, using a combination of mutational approaches, biochemical studies, peptide arrays and plasmon surface resonance (BIAcore), we fine map and characterize a minimal 24 amino acid long RKIP binding domain in the Raf-1 N-region, which consists of constitutive elements a ...[more]
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