Unknown

Dataset Information

0

HnRNP L regulates differences in expression of mouse integrin alpha2beta1.


ABSTRACT: There is a 2-fold variation in platelet integrin alpha2beta1 levels among inbred mouse strains. Decreased alpha2beta1 in 4 strains carrying Itga2 haplotype 2 results from decreased affinity of heterogeneous ribonucleoprotein L (hnRNP L) for a 6 CA repeat sequence (CA6) within intron 1. Seven strains bearing haplotype 1 and a 21 CA repeat sequence at this position (CA21) express twice the level of platelet alpha2beta1 and exhibit an equivalent gain of platelet function in vitro. By UV crosslinking and immunoprecipitation, hnRNP L binds more avidly to CA21, relative to CA6. By cell-free, in vitro mRNA splicing, decreased binding of hnRNP L results in decreased splicing efficiency and an increased proportion of alternatively spliced product. The splicing enhancer activity of CA21 in vivo is abolished by prior treatment with hnRNP L-specific siRNA. Thus, decreased surface alpha2beta1 results from decreased Itga2 pre-mRNA splicing regulated by hnRNP L and depends on CA repeat length at a specific site in intron 1.

SUBMITTER: Cheli Y 

PROVIDER: S-EPMC1895792 | biostudies-literature | 2006 Jun

REPOSITORIES: biostudies-literature

altmetric image

Publications

hnRNP L regulates differences in expression of mouse integrin alpha2beta1.

Cheli Yann Y   Kunicki Thomas J TJ  

Blood 20060202 11


There is a 2-fold variation in platelet integrin alpha2beta1 levels among inbred mouse strains. Decreased alpha2beta1 in 4 strains carrying Itga2 haplotype 2 results from decreased affinity of heterogeneous ribonucleoprotein L (hnRNP L) for a 6 CA repeat sequence (CA6) within intron 1. Seven strains bearing haplotype 1 and a 21 CA repeat sequence at this position (CA21) express twice the level of platelet alpha2beta1 and exhibit an equivalent gain of platelet function in vitro. By UV crosslinkin  ...[more]

Similar Datasets

| S-EPMC3828121 | biostudies-literature
| S-EPMC9353741 | biostudies-literature
| S-EPMC2234046 | biostudies-literature
| S-EPMC3149370 | biostudies-literature
| S-EPMC2808374 | biostudies-literature
| S-EPMC3057834 | biostudies-literature
| S-EPMC2837621 | biostudies-literature
| S-EPMC2731151 | biostudies-literature
| S-EPMC9253288 | biostudies-literature
| S-EPMC6197073 | biostudies-literature