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A p53-type response element in the GDF15 promoter confers high specificity for p53 activation.

ABSTRACT: GDF15 is a transcriptional target gene for p53 and its family members, p63 and p73. Its promoter region contains two p53-type response elements, RE1 and RE2, and RE2 confers p53-specific transactivation. RE2 contains several mismatches from the canonical p53 response element (RRRCWWGYYY). Two mismatches in the RRR span and T base of the RE2 core sequence in the most 3' quarter-site are critical for inhibiting the binding affinity to p63 and p73 and corresponding promoter activity. Our results strongly suggest that differential DNA-binding affinities between p53 family member proteins act, at least in part, to confer specific target gene activation.

SUBMITTER: Osada M

PROVIDER: S-EPMC1975780 | biostudies-literature | 2007 Mar

REPOSITORIES: biostudies-literature

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A p53-type response element in the GDF15 promoter confers high specificity for p53 activation.

Osada Motonobu M Park Hannah Lui HL Park Min Joo MJ Liu Jun-Wei JW Wu Guojun G Trink Barry B Sidransky David D

Biochemical and biophysical research communications 20070125 4

GDF15 is a transcriptional target gene for p53 and its family members, p63 and p73. Its promoter region contains two p53-type response elements, RE1 and RE2, and RE2 confers p53-specific transactivation. RE2 contains several mismatches from the canonical p53 response element (RRRCWWGYYY). Two mismatches in the RRR span and T base of the RE2 core sequence in the most 3' quarter-site are critical for inhibiting the binding affinity to p63 and p73 and corresponding promoter activity. Our results st ...[more]

PMID: 17276395

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