Ontology highlight
ABSTRACT:
SUBMITTER: Collins B
PROVIDER: S-EPMC1978149 | biostudies-literature | 2005 Dec
REPOSITORIES: biostudies-literature
Collins Bernard B Stevens Raymond C RC Page Rebecca R
Acta crystallographica. Section F, Structural biology and crystallization communications 20051105 Pt 12
An optimal solubility screen is described that uses the results of crystallization trials to identify buffers that improve protein solubility and, in turn, crystallization success. This screen is useful not only for standard crystallization experiments, but also can easily be implemented into any high-throughput structure-determination pipeline. As a proof of principle, the predicted novel-fold protein AF2059 from Archaeoglobus fulgidus, which was known to precipitate in most buffers and particu ...[more]