Ontology highlight
ABSTRACT:
SUBMITTER: Guan L
PROVIDER: S-EPMC2000551 | biostudies-literature | 2007 Sep
REPOSITORIES: biostudies-literature
Guan Lan L Mirza Osman O Verner Gillian G Iwata So S Kaback H Ronald HR
Proceedings of the National Academy of Sciences of the United States of America 20070919 39
Here we describe an x-ray structure of wild-type lactose permease (LacY) from Escherichia coli determined by manipulating phospholipid content during crystallization. The structure exhibits the same global fold as the previous x-ray structures of a mutant that binds sugar but cannot catalyze translocation across the membrane. LacY is organized into two six-helix bundles with twofold pseudosymmetry separated by a large interior hydrophilic cavity open only to the cytoplasmic side and containing t ...[more]