Ontology highlight
ABSTRACT:
SUBMITTER: Hariharan P
PROVIDER: S-EPMC6478161 | biostudies-literature | 2016 Oct
REPOSITORIES: biostudies-literature
Hariharan Parameswaran P Andersson Magnus M Jiang Xiaoxu X Pardon Els E Steyaert Jan J Kaback H Ronald HR Guan Lan L
Biochemistry 20161012 42
Camelid nanobodies (Nbs) raised against the outward-facing conformer of a double-Trp mutant of the lactose permease of Escherichia coli (LacY) stabilize the permease in outward-facing conformations. Isothermal titration calorimetry is applied herein to dissect the binding thermodynamics of two Nbs, one that markedly improves access to the sugar-binding site and another that dramatically increases the affinity for galactoside. The findings presented here show that both enthalpy and entropy contri ...[more]