Unknown

Dataset Information

0

Identification of Tam41 maintaining integrity of the TIM23 protein translocator complex in mitochondria.


ABSTRACT: Newly synthesized mitochondrial proteins are imported into mitochondria with the aid of protein translocator complexes in the outer and inner mitochondrial membranes. We report the identification of yeast Tam41, a new member of mitochondrial protein translocator systems. Tam41 is a peripheral inner mitochondrial membrane protein facing the matrix. Disruption of the TAM41 gene led to temperature-sensitive growth of yeast cells and resulted in defects in protein import via the TIM23 translocator complex at elevated temperature both in vivo and in vitro. Although Tam41 is not a constituent of the TIM23 complex, depletion of Tam41 led to a decreased molecular size of the TIM23 complex and partial aggregation of Pam18 and -16. Import of Pam16 into mitochondria without Tam41 was retarded, and the imported Pam16 formed aggregates in vitro. These results suggest that Tam41 facilitates mitochondrial protein import by maintaining the functional integrity of the TIM23 protein translocator complex from the matrix side of the inner membrane.

SUBMITTER: Tamura Y 

PROVIDER: S-EPMC2064306 | biostudies-literature | 2006 Aug

REPOSITORIES: biostudies-literature

altmetric image

Publications

Identification of Tam41 maintaining integrity of the TIM23 protein translocator complex in mitochondria.

Tamura Yasushi Y   Harada Yoshihiro Y   Yamano Koji K   Watanabe Kazuaki K   Ishikawa Daigo D   Ohshima Chié C   Nishikawa Shuh-ichi S   Yamamoto Hayashi H   Endo Toshiya T  

The Journal of cell biology 20060801 5


Newly synthesized mitochondrial proteins are imported into mitochondria with the aid of protein translocator complexes in the outer and inner mitochondrial membranes. We report the identification of yeast Tam41, a new member of mitochondrial protein translocator systems. Tam41 is a peripheral inner mitochondrial membrane protein facing the matrix. Disruption of the TAM41 gene led to temperature-sensitive growth of yeast cells and resulted in defects in protein import via the TIM23 translocator c  ...[more]

Similar Datasets

| S-EPMC2396396 | biostudies-literature
| S-EPMC4648641 | biostudies-literature
| S-EPMC145450 | biostudies-literature
| S-EPMC3243567 | biostudies-literature
| S-EPMC2516936 | biostudies-literature
| S-EPMC5580885 | biostudies-literature
| S-EPMC10079970 | biostudies-literature
| S-EPMC4987294 | biostudies-literature
| S-EPMC3025493 | biostudies-literature
| S-EPMC10718285 | biostudies-literature