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Infection control by antibody disruption of bacterial quorum sensing signaling.


ABSTRACT: Quorum sensing (QS) is the process through which bacteria communicate utilizing small diffusible molecules termed autoinducers. It has been demonstrated that QS controls a plethora of microbial processes including the expression of virulence factors. Here we report an immunopharmacotherapeutic approach for the attenuation of QS in the Gram-positive human pathogen Staphylococcus aureus. An anti-autoinducer monoclonal antibody, AP4-24H11, was elicited against a rationally designed hapten, and efficiently inhibited QS in vitro through the sequestration of the autoinducing peptide (AIP)-4 produced by S. aureus RN4850. Importantly, AP4-24H11 suppressed S. aureus pathogenicity in an abscess formation mouse model in vivo and provided complete protection against a lethal S. aureus challenge. These findings provide a strong foundation for further investigations of immunopharmacotherapy for the treatment of bacterial infections in which QS controls the expression of virulence factors.

SUBMITTER: Park J 

PROVIDER: S-EPMC2088803 | biostudies-literature | 2007 Oct

REPOSITORIES: biostudies-literature

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Infection control by antibody disruption of bacterial quorum sensing signaling.

Park Junguk J   Jagasia Reshma R   Kaufmann Gunnar F GF   Mathison John C JC   Ruiz Diana I DI   Moss Jason A JA   Meijler Michael M MM   Ulevitch Richard J RJ   Janda Kim D KD  

Chemistry & biology 20071001 10


Quorum sensing (QS) is the process through which bacteria communicate utilizing small diffusible molecules termed autoinducers. It has been demonstrated that QS controls a plethora of microbial processes including the expression of virulence factors. Here we report an immunopharmacotherapeutic approach for the attenuation of QS in the Gram-positive human pathogen Staphylococcus aureus. An anti-autoinducer monoclonal antibody, AP4-24H11, was elicited against a rationally designed hapten, and effi  ...[more]

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