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Negamycin binds to the wall of the nascent chain exit tunnel of the 50S ribosomal subunit.

ABSTRACT: Negamycin, a small-molecule inhibitor of protein synthesis, binds the Haloarcula marismortui 50S ribosomal subunit at a single site formed by highly conserved RNA nucleotides near the cytosolic end of the nascent chain exit tunnel. The mechanism of antibiotic action and the function of this unexplored tunnel region remain intriguingly elusive.

SUBMITTER: Schroeder SJ

PROVIDER: S-EPMC2167971 | biostudies-literature | 2007 Dec

REPOSITORIES: biostudies-literature

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Negamycin binds to the wall of the nascent chain exit tunnel of the 50S ribosomal subunit.

Schroeder Susan J SJ Blaha Gregor G Moore Peter B PB

Antimicrobial agents and chemotherapy 20070730 12

Negamycin, a small-molecule inhibitor of protein synthesis, binds the Haloarcula marismortui 50S ribosomal subunit at a single site formed by highly conserved RNA nucleotides near the cytosolic end of the nascent chain exit tunnel. The mechanism of antibiotic action and the function of this unexplored tunnel region remain intriguingly elusive. ...[more]

PMID: 17664317

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