Ontology highlight
ABSTRACT:
SUBMITTER: Zellner E
PROVIDER: S-EPMC2175324 | biostudies-literature | 2007
REPOSITORIES: biostudies-literature
Zellner Elisabeth E Herrmann Thomas T Schulz Carsten C Grummt Friedrich F
Nucleic acids research 20071004 20
Eukaryotic DNA replication initiates at origins of replication by the assembly of the highly conserved pre-replicative complex (pre-RC). However, exact sequences for pre-RC binding still remain unknown. By chromatin immunoprecipitation we identified in vivo a pre-RC-binding site within the origin of bidirectional replication in the murine rDNA locus. At this sequence, ORC1, -2, -4 and -5 are bound in G1 phase and at the G1/S transition. During S phase, ORC1 is released. An ATP-dependent and site ...[more]