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LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling.


ABSTRACT: Lymphocyte membrane rafts contain molecules critical for immunoreceptor signaling. Here, we report identification of a new raft-associated adaptor protein LIME (Lck-interacting molecule) expressed predominantly in T lymphocytes. LIME becomes tyrosine phosphorylated after cross-linking of the CD4 or CD8 coreceptors. Phospho-LIME associates with the Src family kinase Lck and its negative regulator, Csk. Ectopic expression of LIME in Jurkat T cells results in an increase of Csk in lipid rafts, increased phosphorylation of Lck and higher Ca2+ response to CD3 stimulation. Thus, LIME appears to be involved in regulation of T cell activation by coreceptors.

SUBMITTER: Brdickova N 

PROVIDER: S-EPMC2194115 | biostudies-literature | 2003 Nov

REPOSITORIES: biostudies-literature

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LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling.

Brdicková Nadezda N   Brdicka Tomás T   Angelisová Pavla P   Horváth Ondrej O   Spicka Jiri J   Hilgert Ivan I   Paces Jan J   Simeoni Luca L   Kliche Stefanie S   Merten Camilla C   Schraven Burkhart B   Horejsí Václav V  

The Journal of experimental medicine 20031110 10


Lymphocyte membrane rafts contain molecules critical for immunoreceptor signaling. Here, we report identification of a new raft-associated adaptor protein LIME (Lck-interacting molecule) expressed predominantly in T lymphocytes. LIME becomes tyrosine phosphorylated after cross-linking of the CD4 or CD8 coreceptors. Phospho-LIME associates with the Src family kinase Lck and its negative regulator, Csk. Ectopic expression of LIME in Jurkat T cells results in an increase of Csk in lipid rafts, incr  ...[more]

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