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Natural killer T cells infiltrate neuroblastomas expressing the chemokine CCL2.

ABSTRACT: CD1d-restricted Valpha24-Jalpha18-invariant natural killer T cells (iNKTs) are potentially important in tumor immunity. However, little is known about their localization to tumors. We analyzed 98 untreated primary neuroblastomas from patients with metastatic disease (stage 4) for tumor-infiltrating iNKTs using TaqMan((R)) reverse transcription polymerase chain reaction and immunofluorescent microscopy. 52 tumors (53%) contained iNKTs, and oligonucleotide microarray analysis of the iNKT(+) and iNKT(-) tumors revealed that the former expressed higher levels of CCL2/MCP-1, CXCL12/SDF-1, CCL5/RANTES, and CCL21/SLC. Eight tested neuroblastoma cell lines secreted a range of CCL2 (0-21.6 ng/ml), little CXCL12 (

SUBMITTER: Metelitsa LS

PROVIDER: S-EPMC2211904 | biostudies-literature | 2004 May

REPOSITORIES: biostudies-literature

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Natural killer T cells infiltrate neuroblastomas expressing the chemokine CCL2.

Metelitsa Leonid S LS Wu Hong-Wei HW Wang Hong H Yang Yujun Y Warsi Zamir Z Asgharzadeh Shahab S Groshen Susan S Wilson S Brian SB Seeger Robert C RC

The Journal of experimental medicine 20040501 9

CD1d-restricted Valpha24-Jalpha18-invariant natural killer T cells (iNKTs) are potentially important in tumor immunity. However, little is known about their localization to tumors. We analyzed 98 untreated primary neuroblastomas from patients with metastatic disease (stage 4) for tumor-infiltrating iNKTs using TaqMan((R)) reverse transcription polymerase chain reaction and immunofluorescent microscopy. 52 tumors (53%) contained iNKTs, and oligonucleotide microarray analysis of the iNKT(+) and iN ...[more]

PMID: 15123743

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