Ontology highlight
ABSTRACT:
SUBMITTER: Ye JD
PROVIDER: S-EPMC2224236 | biostudies-literature | 2008 Jan
REPOSITORIES: biostudies-literature
Ye Jing-Dong JD Tereshko Valentina V Frederiksen John K JK Koide Akiko A Fellouse Frederic A FA Sidhu Sachdev S SS Koide Shohei S Kossiakoff Anthony A AA Piccirilli Joseph A JA
Proceedings of the National Academy of Sciences of the United States of America 20071227 1
Antibodies that bind protein antigens are indispensable in biochemical research and modern medicine. However, knowledge of RNA-binding antibodies and their application in the ever-growing RNA field is lacking. Here we have developed a robust approach using a synthetic phage-display library to select specific antigen-binding fragments (Fabs) targeting a large functional RNA. We have solved the crystal structure of the first Fab-RNA complex at 1.95 A. Capability in phasing and crystal contact form ...[more]