Ontology highlight
ABSTRACT:
SUBMITTER: Sivaraman K
PROVIDER: S-EPMC2241905 | biostudies-literature | 2008 Feb
REPOSITORIES: biostudies-literature
Sivaraman Karthikeyan K Seshasayee Aswinsainarain A Tarwater Patrick M PM Cole Alexander M AM
Nucleic acids research 20080118 3
Algorithms for theoretical reverse translation have direct applications in degenerate PCR. The conventional practice is to create several degenerate primers each of which variably encode the peptide region of interest. In the current work, for each codon we have analyzed the flanking residues in proteins and determined their influence on codon choice. From this, we created a method for theoretical reverse translation that includes information from flanking residues of the protein in question. Ou ...[more]