Ontology highlight
ABSTRACT:
SUBMITTER: Agrawal A
PROVIDER: S-EPMC2265145 | biostudies-literature | 2008 Mar
REPOSITORIES: biostudies-literature
Agrawal Amit A Deo Rajesh R Wang Geoffrey D GD Wang May D MD Nie Shuming S
Proceedings of the National Academy of Sciences of the United States of America 20080227 9
We report a method for single-molecule detection and biomolecular structural mapping based on dual-color imaging and automated colocalization of bioconjugated nanoparticle probes at nanometer precision. In comparison with organic dyes and fluorescent proteins, nanoparticle probes such as fluorescence energy-transfer nanobeads and quantum dots provide significant advantages in signal brightness, photostability, and multicolor-light emission. As a result, we have achieved routine two-color superre ...[more]