Ontology highlight
ABSTRACT:
SUBMITTER: McCulloch SD
PROVIDER: S-EPMC2288658 | biostudies-literature | 2007 Jul
REPOSITORIES: biostudies-literature
McCulloch Scott D SD Wood Adam A Garg Parie P Burgers Peter M J PM Kunkel Thomas A TA
Biochemistry 20070704 30
Among several hypotheses to explain how translesion synthesis (TLS) by DNA polymerase eta (pol eta) suppresses ultraviolet light-induced mutagenesis in vivo despite the fact that pol eta copies DNA with low fidelity, here we test whether replication accessory proteins enhance the fidelity of TLS by pol eta. We first show that the single-stranded DNA binding protein RPA, the sliding clamp PCNA, and the clamp loader RFC slightly increase the processivity of yeast pol eta and its ability to recycle ...[more]