Vector and parameters for targeted transgenic RNA interference in Drosophila melanogaster.
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ABSTRACT:
SUBMITTER: Ni JQ
PROVIDER: S-EPMC2290002 | biostudies-literature | 2008 Jan
REPOSITORIES: biostudies-literature
Publications
Vector and parameters for targeted transgenic RNA interference in Drosophila melanogaster.
Ni Jian-Quan JQ Markstein Michele M Binari Richard R Pfeiffer Barret B Liu Lu-Ping LP Villalta Christians C Booker Matthew M Perkins Lizabeth L Perrimon Norbert N
Nature methods 20071216 1
The conditional expression of hairpin constructs in Drosophila melanogaster has emerged in recent years as a method of choice in functional genomic studies. To date, upstream activating site-driven RNA interference constructs have been inserted into the genome randomly using P-element-mediated transformation, which can result in false negatives due to variable expression. To avoid this problem, we have developed a transgenic RNA interference vector based on the phiC31 site-specific integration m ...[more]
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