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DCHD3, a novel ATP-dependent chromatin remodeler associated with sites of active transcription.

ABSTRACT: ATP-dependent chromatin remodelers of the CHD family play important roles during differentiation and development. Three CHD proteins, dMi-2, dChd1, and Kismet, have been described for Drosophila melanogaster. Here, we study dCHD3, a novel member of the CHD family. dCHD3 is related in sequence to dMi-2 but lacks several domains implicated in dMi-2 function. We demonstrate that dCHD3 is a nuclear protein and that expression is tightly regulated during fly development. Recombinant dCHD3 remodels mono- and polynucleosomes in an ATP-dependent manner in vitro. Its chromodomains are critical for nucleosome binding and remodeling. Unlike dMi-2, dCHD3 exists as a monomer. Nevertheless, both proteins colocalize with RNA polymerase II to actively transcribed regions on polytene chromosomes, suggesting that both remodelers participate in the process of transcription.

SUBMITTER: Murawska M

PROVIDER: S-EPMC2293103 | biostudies-literature | 2008 Apr

REPOSITORIES: biostudies-literature

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dCHD3, a novel ATP-dependent chromatin remodeler associated with sites of active transcription.

Murawska Magdalena M Kunert Natascha N van Vugt Joke J Längst Gernot G Kremmer Elisabeth E Logie Colin C Brehm Alexander A

Molecular and cellular biology 20080204 8

ATP-dependent chromatin remodelers of the CHD family play important roles during differentiation and development. Three CHD proteins, dMi-2, dChd1, and Kismet, have been described for Drosophila melanogaster. Here, we study dCHD3, a novel member of the CHD family. dCHD3 is related in sequence to dMi-2 but lacks several domains implicated in dMi-2 function. We demonstrate that dCHD3 is a nuclear protein and that expression is tightly regulated during fly development. Recombinant dCHD3 remodels mo ...[more]

PMID: 18250149

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