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Regulation of alternative polyadenylation by genomic imprinting.

ABSTRACT: Maternally and paternally derived alleles can utilize different promoters, but allele-specific differences in cotranscriptional processes have not been reported. We show that alternative polyadenylation sites at a novel murine imprinted gene (H13) are utilized in an allele-specific manner. A differentially methylated CpG island separates polyA sites utilized on maternal and paternal alleles, and contains an internal promoter. Two genetic systems show that alleles lacking methylation generate truncated H13 transcripts that undergo internal polyadenylation. On methylated alleles, the internal promoter is inactive and elongation proceeds to downstream polyadenylation sites. This demonstrates that epigenetic modifications can influence utilization of alternative polyadenylation sites.

SUBMITTER: Wood AJ

PROVIDER: S-EPMC2335310 | biostudies-literature | 2008 May

REPOSITORIES: biostudies-literature

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Regulation of alternative polyadenylation by genomic imprinting.

Wood Andrew J AJ Schulz Reiner R Woodfine Kathryn K Koltowska Katarzyna K Beechey Colin V CV Peters Jo J Bourc'his Deborah D Oakey Rebecca J RJ

Genes & development 20080501 9

Maternally and paternally derived alleles can utilize different promoters, but allele-specific differences in cotranscriptional processes have not been reported. We show that alternative polyadenylation sites at a novel murine imprinted gene (H13) are utilized in an allele-specific manner. A differentially methylated CpG island separates polyA sites utilized on maternal and paternal alleles, and contains an internal promoter. Two genetic systems show that alleles lacking methylation generate tru ...[more]

PMID: 18451104

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