Ontology highlight
ABSTRACT:
SUBMITTER: Lewinson O
PROVIDER: S-EPMC2362151 | biostudies-literature | 2008 Mar
REPOSITORIES: biostudies-literature
Journal of molecular biology 20080104 1
Challenges in the production of integral membrane proteins for structural studies include low expression levels, incorrect membrane insertion, aggregation and instability. In this report, we describe a "funnel approach" to overcoming these difficulties and demonstrate its efficacy in a case study of 36 prokaryotic P-type transporters. A diverse ensemble of modified constructs is generated and tested for expression in Escherichia coli, membrane localization, detergent extraction, and homogeneity. ...[more]