Ontology highlight
ABSTRACT:
SUBMITTER: Russell RS
PROVIDER: S-EPMC2393785 | biostudies-literature | 2008 Mar
REPOSITORIES: biostudies-literature
Russell Rodney S RS Meunier Jean-Christophe JC Takikawa Shingo S Faulk Kristina K Engle Ronald E RE Bukh Jens J Purcell Robert H RH Emerson Suzanne U SU
Proceedings of the National Academy of Sciences of the United States of America 20080311 11
The JFH1 strain of hepatitis C virus (HCV) is unique among HCV isolates, in that the wild-type virus can traverse the entire replication cycle in cultured cells. However, without adaptive mutations, only low levels of infectious virus are produced. In the present study, the effects of five mutations that were selected during serial passage in Huh-7.5 cells were studied. Recombinant genomes containing all five mutations produced 3-4 logs more infectious virions than did wild type. Neither a codin ...[more]