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A curved RNA helix incorporating an internal loop with G.A and A.A non-Watson-Crick base pairing.

ABSTRACT: The crystal structure of the RNA dodecamer 5'-GGCC(GAAA)GGCC-3' has been determined from x-ray diffraction data to 2.3-A resolution. In the crystal, these oligomers form double helices around twofold symmetry axes. Four consecutive non-Watson-Crick base pairs make up an internal loop in the middle of the duplex, including sheared G.A pairs and novel asymmetric A.A pairs. This internal loop sequence produces a significant curvature and narrowing of the double helix. The helix is curved by 34 degrees from end to end and the diameter is narrowed by 24% in the internal loop. A Mn2+ ion is bound directly to the N7 of the first guanine in the Watson-Crick region following the internal loop and the phosphate of the preceding residue. This Mn2+ location corresponds to a metal binding site observed in the hammerhead catalytic RNA.

SUBMITTER: Baeyens KJ

PROVIDER: S-EPMC24009 | biostudies-literature | 1996 Nov

REPOSITORIES: biostudies-literature

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A curved RNA helix incorporating an internal loop with G.A and A.A non-Watson-Crick base pairing.

Baeyens K J KJ De Bondt H L HL Pardi A A Holbrook S R SR

Proceedings of the National Academy of Sciences of the United States of America 19961101 23

The crystal structure of the RNA dodecamer 5'-GGCC(GAAA)GGCC-3' has been determined from x-ray diffraction data to 2.3-A resolution. In the crystal, these oligomers form double helices around twofold symmetry axes. Four consecutive non-Watson-Crick base pairs make up an internal loop in the middle of the duplex, including sheared G.A pairs and novel asymmetric A.A pairs. This internal loop sequence produces a significant curvature and narrowing of the double helix. The helix is curved by 34 degr ...[more]

PMID: 8917508

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