Ontology highlight
ABSTRACT:
SUBMITTER: Baumann P
PROVIDER: S-EPMC24327 | biostudies-literature | 1998 Nov
REPOSITORIES: biostudies-literature
Proceedings of the National Academy of Sciences of the United States of America 19981101 24
Mammalian cells defective in DNA end-joining are highly sensitive to ionizing radiation and are immunodeficient because of a failure to complete V(D)J recombination. By using cell-free extracts prepared from human lymphoblastoid cell lines, an in vitro system for end-joining has been developed. Intermolecular ligation was found to be accurate and to depend on DNA ligase IV/Xrcc4 and requires Ku70, Ku86, and DNA-PKcs, the three subunits of the DNA-activated protein kinase DNA-PK. Because these ac ...[more]