Genetic differentiation of Escherichia coli O157:H7 clades associated with human disease by real-time PCR.
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ABSTRACT:
SUBMITTER: Riordan JT
PROVIDER: S-EPMC2446876 | biostudies-literature | 2008 Jun
REPOSITORIES: biostudies-literature
Publications
Genetic differentiation of Escherichia coli O157:H7 clades associated with human disease by real-time PCR.
Riordan James T JT Viswanath Sandeep B SB Manning Shannon D SD Whittam Thomas S TS
Journal of clinical microbiology 20080409 6
The rapid and accurate identification of Escherichia coli O157:H7 strains is central to reducing the impact of outbreaks. A real-time PCR-based approach to differentiating major outbreak lineages of O157 with novel single-nucleotide polymorphisms is described. The utility of this method is in detection of hypervirulent strains in cases of clinical disease. ...[more]
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