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Footprinting analysis demonstrates extensive similarity between eukaryotic RNase P and RNase MRP holoenzymes.

ABSTRACT: Eukaryotic ribonuclease (RNase) P and RNase MRP are evolutionary related RNA-based enzymes involved in metabolism of various RNA molecules, including tRNA and rRNA. In contrast to the closely related eubacterial RNase P, which is comprised of an RNA component and a single small protein, these enzymes contain multiple protein components. Here we report the results of footprinting studies performed on purified Saccharomyces cerevisiae RNase MRP and RNase P holoenzymes. The results identify regions of the RNA components affected by the protein moiety, suggest a role of the proteins in stabilization of the RNA fold, and point to substantial similarities between the two evolutionary related RNA-based enzymes.

SUBMITTER: Esakova O

PROVIDER: S-EPMC2491465 | biostudies-literature | 2008 Aug

REPOSITORIES: biostudies-literature

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Footprinting analysis demonstrates extensive similarity between eukaryotic RNase P and RNase MRP holoenzymes.

Esakova Olga O Perederina Anna A Quan Chao C Schmitt Mark E ME Krasilnikov Andrey S AS

RNA (New York, N.Y.) 20080625 8

Eukaryotic ribonuclease (RNase) P and RNase MRP are evolutionary related RNA-based enzymes involved in metabolism of various RNA molecules, including tRNA and rRNA. In contrast to the closely related eubacterial RNase P, which is comprised of an RNA component and a single small protein, these enzymes contain multiple protein components. Here we report the results of footprinting studies performed on purified Saccharomyces cerevisiae RNase MRP and RNase P holoenzymes. The results identify regions ...[more]

PMID: 18579867

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