The RNA-binding protein TbDRBD3 regulates the stability of a specific subset of mRNAs in trypanosomes.

ABSTRACT: In trypanosomes, the apparent lack of regulation of RNA polymerase II-dependent transcription initiation poses a challenge to understand how these eukaryotes adjust gene expression to adapt to the contrasting environments they find during their life cycles. Evidence so far indicates that mRNA turnover and translation are the major control points in which regulation is exerted in trypanosomes. However, very little is known about which proteins are involved, and how do they regulate the abundance and translation of different mRNAs in different life stages. In this work, an RNA-binding protein, TbDRBD3, has been identified by affinity chromatography, and its function addressed using RNA interference, microarray analysis and immunoprecipitation of mRNA-protein complexes. The results obtained i

SUBMITTER: Estevez AM 

PROVIDER: S-EPMC2504296 | biostudies-literature | 2008 Aug

REPOSITORIES: biostudies-literature