Ontology highlight
ABSTRACT:
SUBMITTER: Yang F
PROVIDER: S-EPMC2516346 | biostudies-literature | 2007 Nov
REPOSITORIES: biostudies-literature
Yang Feng F Jaitly Navdeep N Jayachandran Hemalatha H Luo Quanzhou Q Monroe Matthew E ME Du Xiuxia X Gritsenko Marina A MA Zhang Rui R Anderson David J DJ Purvine Samuel O SO Adkins Joshua N JN Moore Ronald J RJ Mottaz Heather M HM Ding Shi-Jian SJ Lipton Mary S MS Camp David G DG Udseth Harold R HR Smith Richard D RD Rossie Sandra S
Journal of proteome research 20071012 11
To identify phosphoproteins regulated by the phosphoprotein phosphatase (PPP) family of S/T phosphatases, we performed a large-scale characterization of changes in protein phosphorylation on extracts from HeLa cells treated with or without calyculin A, a potent PPP enzyme inhibitor. A label-free comparative phosphoproteomics approach using immobilized metal ion affinity chromatography and targeted tandem mass spectrometry was employed to discover and identify signatures based upon distinctive ch ...[more]