Ontology highlight
ABSTRACT:
SUBMITTER: Pant K
PROVIDER: S-EPMC2527458 | biostudies-literature | 2008 Jul
REPOSITORIES: biostudies-literature
Pant Kiran K Shokri Leila L Karpel Richard L RL Morrical Scott W SW Williams Mark C MC
Journal of molecular biology 20080524 5
Bacteriophage T4 UvsY is a recombination mediator protein that promotes assembly of the UvsX-ssDNA presynaptic filament. UvsY helps UvsX to displace T4 gene 32 protein (gp32) from ssDNA, a reaction necessary for proper formation of the presynaptic filament. Here we use DNA stretching to examine UvsY interactions with single DNA molecules in the presence and absence of gp32 and a gp32 C-terminal truncation (*I), and show that in both cases UvsY is able to destabilize gp32-ssDNA interactions. In t ...[more]